#Z087. Taq antibody
A. Description
- Taq antibody is a monoclonal antibody that binds Taq DNA polymerase and inhibits its activity until reaction temperature is elevated. At that point, the Taq antibody is denatured and releases its hold on Taq DNA polymerase, allowing DNA synthesis to proceed. The binding of the Taq antibody to Taq DNA polymerase is the premise of hot-start PCR.
- It is used in the preparation of hot-start PCR or qPCR master mixes and helps to prevent nonspecific amplification and primer-dimer formation, increasing specificity and sensitivity while allowing assembly of reactions at ambient temperatures. At the first denaturation step of the thermal cycling, the antibody is quickly inactivated and PCR proceeds. The antibody-mediated hot start method is significantly more convenient to use than other hot start methods.
B. Features
- Enhances the specificity and sensitivity of PCR.
- Can be used when other hot start methods are difficult to perform (e.g. capillary PCR).
- The polymerase can be reactivated quicker than with methods utilizing a chemically modified polymerase.
C. Quality Control
After multiple column purifications, only a clear single target band was visible in SDS-PAGE gel detection, and the purity is 95%; the test showed no RNase, endonuclease or exonuclease contamination.
D. Storage -20℃.
E. Components
| Component | Z087-01A | Z087-01B | Z087-M001 | Z087-M200 | Z087-HC01-M001 | Z087-HC02-M001 |
| Taq antibody (5U/μl) | 50μl | 50μl×5 | 1ml | 200ml | - | - |
| Taq antibody (20U/μl) | - | - | - | - | 1ml | - |
| Taq antibody (40U/μl) | - | - | - | - | - | 1ml |
F. ctivity Definition
The amount of Taq antibody required to inhibit more than 95% of 1U Taq DNA polymerase activity at 55℃ for 30min is defined as 1U.
G. Notes
The amount of Taq antibody required to inhibit more than 95% of 1U Taq DNA polymerase activity at 55℃ for 30min is defined as 1U.
G. Notes
- Mix Taq DNA polymerase and Taq antibody with the same volume, and place it at 20-25℃ for about 30min.
- The PCR reaction according to various PCR reaction conditions of Taq DNA polymerase. It is confirmed by experiments that Taq antibody can be used in conjunction with Taq DNA polymerase (Cat# E001) and the recommended ratio is 1U : 1U.
| Cat# | Product Names |
| E001 | Taq DNA Polymerase |
| E063 | Heat-labile Uracil-DNA Glycosylase |
| E060 | Uracil-DNA Glycosylase (UDG) |
| E108 | HotStart Tth DNA Polymerase |
| M001 | T4 DNA Ligase |
| M005 | T4 DNA Polymerase |
| M007 | Exonuclease III |
| E097 | HotStart Taq DNA Polymerase (B) |
| E017. | HotStart Taq DNA Polymerase |
| M031 | Thermostable Inorganic Pyrophosphatase |
| E127 | DNase I |
| E125 | Recombinant RNase Inhibitor (Murine) |
| E086-YSAA | 5×Multiplex PCR Mix |