#C746, Recombinant Mouse IFN gamma (E. coli)
Recombinant Mouse Interferon Gamma is produced by our E.coli expression system and the target gene encoding His23-Cys155 is expressed.
- Accession: P01580
- Known As: Ifng;Interferon gamma; IFN-gamma
- Mol Mass: 15.7 KDa
- APMol Mass: 14 KDa, reducing conditions
- Formulation Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 5% Sucrose, pH 6.8.
- Endotoxin: Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
- Bioactivity:
Determined by its ability to inhibit the proliferation of murine WEHI-279 cells. The expected ED50 is 1 ng/ml.
- Reconstitution
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in 4mM HCl.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in 4mM HCl.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Shipping
The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.
- Background
Mouse Ifng is a secreted protein which belongs to the type I I (or gamma) interferon family. IFNG is produced by lymphocytes and activated by specific antigens or mitogens. In addition to having antiviral activity, IFNG also has important immunoregulatory functions. It is a potent activator of macrophages and has antiproliferative effects on transformed cells. It can potentiate the antiviral and antitumor effects of the type I interferons. Genetic variation in IFNG is associated with the risk of aplastic anemia (AA) which is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
For Research or Manufacturing Use Only, Not for Use in Diagnostic or Therapeutic Procedures.