Stem Cell Products

#C046, Recombinant Human FGFb (157aa)

#C046, Recombinant Human FGFb (157aa)

FGF-basic is a members of the Fibroblast Growth Factors (FGFs) family.
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詳細介紹

#C046, RECOMBINANT HUMAN FGFB (157AA)

Recombinant Human Fibroblast Growth Factor 2/Fibroblast Growth Factor Basic is produced by our E.coli expression system and the target gene encoding Gly132-Ser288 is expressed.

  • Accession:  P09038
  • Known As: Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGF
  • Mol Mass: 17.4 KDa
  • APMol Mass: 16 KDa, reducing conditions
  • Formulation Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.
  • Endotoxin: Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
  • Purity
Greater than 95% as determined by reducing SDS-PAGE.
  • Bioactivity

 Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 1.11 ng/ml.

  • Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

  • Storage

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

  • Shipping
The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.
  • Background
FGF-basic is a members of the Fibroblast Growth Factors (FGFs) family .The family constitutes a large family of proteins involved in many aspects of development including cell proliferation, growth, and differentiation. They act on several cell types to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects, and tissue repair. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4.
 
For Research or Manufacturing Use Only, Not for Use in Diagnostic or Therapeutic Procedures.